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ROBERT A. EVANGELISTA
BIOCHEMICAL ENGINEER & CHEMIST FOR BIOFUELS & THE ENVIRONMENT

BIOREMEDIATION of PHENOL and CRESOLS

Introduction. The Poly Carb site had phenol- and cresols-contaminated soil. The objective was to explore the use of innovative on-site technologies as an alternative to the transportation and off-site disposal of soil.

Biodegradation. Shake flask tests with genetically-enhanced microorganisms appeared promising to biodegrade soil-bound phenol and cresols. However, Evangelista created soil column studies using Poly Carb soil that showed the addition of these “supercharged bugs” did not improve biodegradation compared to columns containing only indigenous soil microbes. These indigenous microbes were well adapted to degrade soil contaminants. Efforts then focused on enhancing the biological activity of these “local” microbes to remove phenol and cresols from soil.

More Information. Successful Onsite Treatment of Phenols, Cresols. R. Mandel, H. Allen, R. Evangelista, and R. Worobel.
PDF 1.4 MB

Treatment of Phenol and Cresol Contaminated Soil. R. Evangelista, H. Allen, and R. Mandel. Journal of Hazardous Materials, 25:343-60..
PDF 1.1 MB

Soil Remediation Bench-Scale Engineering Studies, Poly-Carb Site, Wells, NV.  R. Evangelista. Prepared for the U.S. Environmental Protection Agency.
PDF 4.6 MB

Evaporation, Soil Washing, and Biodegradation Studies for On-Site Treatment of Phenol and Cresol Contaminated Soil. R. Evangelista, H. Allen, and R. Mandel. American Institute of Chemical Engineers Summer National Meeting Conference Proceedings, Dallas, TX.

In Situ Treatment of Contaminated Soil by Evaporation, Soil Washing and Biodegradation, Wells, NV. H. Allen, R. Mandel, and R. Evangelista. Hazardous Materials Spills Conference Proceedings, Chicago, IL. PDF 2.0 MB

Soil Treatability Studies for Poly-Carb Site, Wells, NV. R. Evangelista. Prepared for the U.S. Environmental Protection Agency.
PDF 2.2 MB

(Mouse over the ThumbNails to see an enlargement)

Biodegradationcartoon SoilcolumnsBiodegradation2a Phenolsoilcolumnresults3a Cresolsoilcolumnsresults4a

Results: Cresols

Results: Phenol

Soil columns where
"bugs" and toxics meet

Toxics eating "SuperBug"

Biodegradation
SoilcolumnsBiodegradation2
Phenolsoilcolumnresults3
Cresolsoilcolumnsresults4
 

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biodiesel, batch

 

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interceptor trench

 

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